IL1R2 Protein, Human, Recombinant (hFc Tag)

• Background:

  Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is a cytokine receptor that belongs to the interleukin-1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. The pleiotropic cytokine IL1 is produced to regulate the development and maintenance of the inflammatory responses and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors that can bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB) . IL1R1 contributes to IL-1 signaling, whereas the IL-1R2/CD121b has no signaling property and acts as a decoy for IL-1. IL-1R2/CD121b structurally consisting of a ligand-binding portion comprised of three Ig-like domains, a single transmembrane region, and a short cytoplasmic domain is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine.

• Synonyms:

  CD121b Protein, Human; CDw121b Protein, Human; IL-1R-2 Protein, Human; IL-1RT-2 Protein, Human; IL-1RT2 Protein, Human; IL1R2c Protein, Human; IL1RB Protein, Human

• Gene Name:

  Interleukin 1 receptor, type II

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  Neuroinflammation

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human IL1B at 2 μg/ml (100ul/well) can bind recombinant human IL1R2-Fc with a linear range of 6.25-400 ng/ml. 2. Using the Octet RED System, the affinity constant (Kd) of IL1R2 Protein, Human, Recombinant (Fc Tag) (Cat. PH50873M4) bound IL-1 beta Protein, Human, Recombinant (Cat. 10139-HNAE) was 6.0 nM. 3. Measured by its ability to inhibit Interferon gamma secretion by human natural killer lymphoma NK-92 cells in the presence of 500 pg/mL IL1b. The ECfor this effect is typically 0.1-0.8 μg/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Interleukin proteins, CD Molecule proteins, Receptor Proteins

• Molecular Weight:

  The recombinant human IL1R2/Fc is a disulfide-linked homodimer. The reduced monomer consists of 568 amino acids and has a predicted molecular mass of 64.5 kDa. As a result of glycosylation, the rh IL1R2/Fc monomer migrates as an approximately 70-75 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  IL1R2

• Species:

  Human

• Protein ID:

  NP_004624.1

• Overview:

  A DNA sequence encoding the extracellular domain (Met 1-Glu 343) of human IL1 receptor type I I (IL1RII) (NP_004624.1) was expressed with C-terminal fused Fc region of human IgG1.

• AA Sequence:

  Met1-Glu343

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_004624.1