CSF1R Protein, Human, Recombinant

• Background:

  M-CSFR encoded by the proto-oncogene c-fms is the receptor for colony stimulating factor 1 (CSF1R), a cytokine involved in the proliferation, differentiation, and activation of macrophages. This cell surface glycoprotein is consisted by an extracellular ligand-binding domain, a single membrane-spanning segment, and an intracellular tyrosine kinase domain. Binding of CSF1 activates the receptor kinase, leading to "autophosphorylation" of receptor subunits and the concomitant phosphorylation of a series of cellular proteins on tyrosine residues. CSF1R is a tyrosine kinase receptor that is absolutely required for macrophage differentiation and thus occupies a central role in hematopoiesis. CSF1 and its receptor (CSF1R, product of c-fms proto-oncogene) were initially implicated as essential for normal monocyte development as well as for trophoblastic implantation. This apparent role for CSF1/CSF1R in normal mammary gland development is very intriguing because this receptor/ligand pair has also been found to be important in the biology of breast cancer in which abnormal expression of CSF1 and its receptor correlates with tumor cell invasiveness and adverse clinical prognosis. Tumor cell expression of CSF1R is under the control of several steroid hormones (glucocorticoids and progestins) and the binding of several bHLH transcription factors, while tumor cell expression of CSF-1 appears to be regulated by other hormones, some of which are involved in normal lactogenic differentiation. However, studies have demonstrated that CSF1 and CSF1R have additional roles in mammary gland development during pregnancy and lactation. The role of CSF1 and CSF1R in normal and neoplastic mammary development that may elucidate potential relationships of growth factor-induced biological changes in the breast during pregnancy and tumor progression.

• Synonyms:

  C-FMS Protein, Human; CD115 Protein, Human; CSF-1R Protein, Human; CSFR Protein, Human; FIM2 Protein, Human; FMS Protein, Human; HDLS Protein, Human; M-CSF-R Protein, Human; MCSF Receptor Protein, Human

• Gene Name:

  Colony stimulating factor 1 receptor

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Field of Research:

  Receptor Tyrosine Kinases (RTKs) Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  1. Immobilized sMCSFR at 10 μg/ml (100 μl/well) can bind biotinylated human CSF1-His (Cat:PH50423M2), The ECof biotinylated human CSF1-His (Cat:PH50423M2) is 32-74.8 ng/ml. 2. Measured by its ability to inhibit the human CSF-induced proliferation of M NFS 60 mouse myelogenous leukemia lymphoblast cells. The EDfor this effect is typically 0.3-1.5 μg/mL in the presence of 3 ng/mL of rhM-CSF.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Biomarker Proteins, Cytokine Proteins

• Molecular Weight:

  The recombinant mature human M-CSF R comprises 499 amino acids with a calculated molecular mass of 55.1 kDa. Due to glycosylation, the apparent molecular mass of rhM-CSFR is approximately 80-90 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CSF1R

• Species:

  Human

• Protein ID:

  NP_005202.2

• Overview:

  The mature form of human M-CSFR (NP_005202.2) extracellular domain (Met 1-Glu 512) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.

• AA Sequence:

  Met1-Glu512

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_005202.2