LDLR/LDL Receptor Protein, Human, Recombinant (His Tag), Biotinylated

• Background:

  LDL Receptor, also known as LDLR, is a mosaic protein that belongs to the Low-density lipoprotein receptor gene family. The low-density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. LDL Receptor consists of 840 amino acids (after removal of signal peptide) and mediates the endocytosis of cholesterol-rich LDL. Low-density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. LDL Receptor is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles. The receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants.

• Synonyms:

  FH Protein, Human; FHC Protein, Human; LDL R Protein, Human; LDL Receptor Protein, Human; LDLCQ2 Protein, Human

• Gene Name:

  Low density lipoprotein receptor

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measure by its ability to bind with human PCSK9 in a functional ELISA. 1. Immobilized human PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The ECof biotinylated human LDLR is 0.61 μg/ml. 2. Immobilized mouse PCSK9 at 10 μg/ml (100 μl/well) can bind biotinylated recombinant human LDLR. The ECof biotinylated human LDLR is 0.12 μg/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Biotinylated proteins

• Molecular Weight:

  The recombinant human LDLR consists of 777 amino acids and predicts a molecular mass of 86 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  LDLR

• Species:

  Human

• Protein ID:

  NP_000518.1

• Overview:

  A DNA sequence encoding the extracellular domain of human LDLR (NP_000518.1) precursor (Met1-Arg788) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.

• AA Sequence:

  Met1-Arg788

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_000518.1