NNT1 Protein, Human, Recombinant (hFc Tag)

• Synonyms:

  BSF-3 Protein, Human; BSF3 Protein, Human; CISS2 Protein, Human; CLC Protein, Human; NNT-1 Protein, Human; NNT1 Protein, Human; NR6 Protein, Human

• Gene Name:

  Cardiotrophin-like cytokine factor 1

• Expression System:

  HEK293 Cells

• Tag:

  N-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Interleukin proteins, Biological Target proteins

• Molecular Weight:

  The recombinant human CLCF1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 458 amino acids and has a predicted molecular mass of 50.7 kDa. The apparent molecular mass of the protein is approximately 54 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CLCF1

• Species:

  Human

• Protein ID:

  NP_037378.1

• Overview:

  A DNA sequence encoding the human CLCF1 (NP_037378.1) (Leu28-Phe225) was expressed with the fused Fc region of human IgG1 at the N-terminus.

• AA Sequence:

  Leu28-Phe225

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_037378.1