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IL-12 Protein, Human, Recombinant

CAT: 0882-PH50280M0-01Size: 10 µgDry Ice: NoHazardous: No
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CAT#:0882-PH50280M0-01Size:10 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Overview
A DNA sequence encoding the human IL12A (P29459-1) (Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2) (Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified.
Synonyms
CLMF Protein, Human; CLMF2 Protein, Human; IL-12A Protein, Human; IL-12B Protein, Human; IL12 Protein, Human; IL12 p70 Protein, Human; IMD28 Protein, Human; IMD29 Protein, Human; NFSK Protein, Human; NKSF Protein, Human; NKSF1 Protein, Human; NKSF2 Protein, Human; P35 Protein, Human
Gene Name
Interleukin 12A
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The EDfor this effect is 0.2-2 ng/mL.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Biological Target proteins, Cytokine Proteins, Growth Factor proteins, Interleukin proteins
Molecular Weight
The recombinant heterodimer of human IL12A&IL12B comprises 503 (197+306) amino acids and has a calculated molecular mass of 57.2 (22.5+34.7) KDa. The apparent molecular mass of human IL12 heterodimer is approximately 36 & 42.4 kDa respectively in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
IL12A & IL12B
Species
Human
Protein ID
P29459-1&NP_002178.2
AA Sequence
Met1-Ser219 & Met1-Ser328
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/P29459-1&NP_002178.2

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