Carbonic Anhydrase IV Protein, Human, Recombinant (His Tag)

• Background:

  The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in COhydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

• Synonyms:

  CAIV Protein, Human; Car4 Protein, Human; RP17 Protein, Human

• Gene Name:

  Carbonic anhydrase IV

• Expression System:

  CHO Stable Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant human CA4 consists of 276 amino acids after removal of the signal peptide and has a predicted molecular mass of 31.7 kDa. In SDS-PAGE under reducing conditions, rh CA4 migrates as approximately 34 and 19 kDa band.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CA4

• Species:

  Human

• Protein ID:

  NP_000708.1

• Overview:

  A DNA sequence encoding the human CA4 (NP_000708.1) (Met1-Lys283) without the pro peptide was expressed with a polyhistidine tag at the C-terminus.

• AA Sequence:

  Met1-Lys283

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_000708.1