Carbonic anhydrase II Protein, Mouse, Recombinant (His Tag)

• Background:

  The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

• Synonyms:

  AI131712 Protein, Mouse; Ca2 Protein, Mouse; CAII Protein, Mouse; Car-2 Protein, Mouse; Ltw-5 Protein, Mouse; Lvtw-5 Protein, Mouse

• Gene Name:

  Carbonic anhydrase II

• Expression System:

  E. coli

• Tag:

  C-His

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 96 % as determined by SDS-PAGE.

• Activity:

  Measured by its esterase activity. The specific activity is >100 pmoles/min/μg.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Enzyme Proteins

• Molecular Weight:

  The recombinant mouse CA2 comprises 270 amino acids and has a calculated molecular mass of 30.4 kDa. The recombinant protein migrates as an approximately 30-33 kDa band in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CA2

• Species:

  Mouse

• Protein ID:

  AAH55291.1

• Overview:

  A DNA sequence encoding the mouse CA2 (AAH55291.1) (Met 1-Lys 260) was expressed, with a C-terminal polyhistidine tag.

• AA Sequence:

  Met1-Lys260

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAH55291.1