Anti-Hu CX3CR1 PE-Cy™7

• Description:

  CX3CR1 (fractalkine receptor) serves as a regulator of leukocyte trafficking. Its ligand CX3CL1 (fractalkine) can serve bots as chemoatractant (soluble) and as adhesive molecule (membrane bound) . Binding to the cell surface expressed ligand leads to rapid induction of firm adhesion of CX3CR1-positive cells without requiring selectin-mediated rolling or activation of integrins. This interaction is important for trafficking of cytotoxic effector lymphocytes regardless of their lineage and mode of target cell recognition, through inflamed endothelium. Similarly, it mediates monocyte/macrophage recruitment to inflamed atherosclerotic plaques, as well as in their homing to non-inflamed tissues, where they can differentiate into dendritic cells. CX3CR1 also promotes cell survival, plays a key role in brain microglia, synapse maturation and regulation of inflammatory response in the central nervous system, it acts as a negative regulator of angiogenesis, and serves as an important regulator of the gut microbiota by controlling immunity to intestinal bacteria and fungi.

• Certification:

  RUO

• Immunogen:

  Human CX3CR1 transfectants

• Target Antigen:

  CX3CR1

• Clone:

  2A9-1

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™7

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Nishimura M, Umehara H, Nakayama T, Yoneda O, Hieshima K, Kakizaki M, Dohmae N, Yoshie O, Imai T: Dual functions of fractalkine/CX3C ligand 1 in trafficking of perforin+/granzyme B+ cytotoxic effector lymphocytes that are defined by CX3CR1 expression. J Immunol. 2002 Jun 15;168 (12) :6173-80., URL: https://pubmed.ncbi.nlm.nih.gov/12055230/, *Nanki T, Imai T, Nagasaka K, Urasaki Y, Nonomura Y, Taniguchi K, Hayashida K, Hasegawa J, Yoshie O, Miyasaka N: Migration of CX3CR1-positive T cells producing type 1 cytokines and cytotoxic molecules into the synovium of patients with rheumatoid arthritis. Arthritis Rheum. 2002 Nov;46 (11) :2878-83. , URL: https://pubmed.ncbi.nlm.nih.gov/12428227/, *Béziat V, Duffy D, Quoc SN, Le Garff-Tavernier M, Decocq J, Combadière B, Debré P, Vieillard V: CD56brightCD16+ NK cells: A functional intermediate stage of NK cell differentiation. J Immunol. 2011 Jun 15;186 (12) :6753-61., URL: https://pubmed.ncbi.nlm.nih.gov/21555534/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody 2A9-1 recognizes an extracellular epitope of CX3CR1, an important regulator of leukocyte trafficking and immune system ballance.

• Applications Notes:

  6

• Species:

  Human, Non-human primates

• Isotype:

  Rat IgG2b kappa