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Human KRT33A (Keratin 33A) Microsample ELISA Kit

CAT: 0855-ELK6748MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6748MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
KRTHA3A; Ha-3I; Krt1-3; K33A; HHA3-I; HKA3A; Keratin, Hair, Acidic 3A; Hard Keratin Type I 3I; Keratin, type I cuticular Ha3-I
UniProt
O76009
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT33A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT33A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT33A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT33A in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
78.13-5000 pg/mL
Sensitivity
29 pg/mL

UniProtKB · O76009

Keratin, type I cuticular Ha3-I

KT33A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O76009
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT33A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2RA87, Q6NTB9, Q6ZZB9
Protein keywords

Domain

Coiled coil

Cellular component

Intermediate filamentKeratin

Technical term

Proteomics identificationReference proteome

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