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Human KRT6A (Keratin 6A) Microsample ELISA Kit

CAT: 0855-ELK4079MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4079MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
K6A; KRT6C; KRT6D; CK6C; K6C; CK6D; K6D; Keratin 6C; Keratin 6D; Keratin, type II cytoskeletal 6A; Type-II keratin Kb6
UniProt
P02538
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT6A in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.6 pg/mL

UniProtKB · P02538

Keratin, type II cytoskeletal 6A

K2C6A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02538
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KRT6A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A4QPC1, P48667, Q08AR4, Q6NT67, Q96CL4
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Acetylation

Disease

AllergenDisease variantEctodermal dysplasiaPalmoplantar keratoderma

Domain

Coiled coil

Cellular component

CytoplasmIntermediate filamentKeratin

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