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Human DMKN (Dermokine) Microsample ELISA Kit

CAT: 0855-ELK6679MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6679MS-01Size:48 Tests
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Product Name Alternative
Epidermis-specific secreted protein SK30/SK89
UniProt
Q6E0U4
Reactivity
Human
Field of Research
Cytokine
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DMKN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DMKN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DMKN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DMKN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.056 ng/mL

UniProtKB · Q6E0U4

Dermokine

DMKN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6E0U4
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DMKN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A3EZ79, A3EZ80, A3EZ81, A3EZ82, A3EZ83, C9J4P6, C9J5N8, C9JAL3, Q32W58, Q32W62, Q32W63, Q32W64, Q32W65, Q32W66, Q32W67, Q6E0U5, Q6UXC7, Q96EW8, Q9BSY6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Glycoprotein

Cellular component

Secreted

Domain

Signal

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