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Human JAM3 (Junctional Adhesion Molecule 3) Microsample ELISA Kit

CAT: 0855-ELK6594MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6594MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
JAM-C; JAMC
UniProt
Q9BX67
Reactivity
Human
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JAM3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JAM3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JAM3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JAM3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
15.63-1000 pg/mL
Sensitivity
6.5 pg/mL

UniProtKB · Q9BX67

Junctional adhesion molecule C

JAM3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BX67
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
JAM3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
JAM-C
EC number
—
Processing
Precursor
Secondary accessions
B3KWG9, Q8WWL8, Q96FL1
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

AngiogenesisCell adhesionDifferentiationSpermatogenesis

Cellular component

Cell junctionCell membraneMembraneSecretedTight junction

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variant

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

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