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Human JAM1 (Junctional Adhesion Molecule 1) Microsample ELISA Kit

CAT: 0855-ELK3922MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3922MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD321; F11R; KAT; JAM-A; JAMA; JCAM; PAM1; Coagulation Factor XI Receptor; Platelet F11 receptor; Platelet adhesion molecule 1
UniProt
Q9Y624
Reactivity
Human
Field of Research
CD & Adhesion molecule; Tumor immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JAM1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.132 ng/mL

UniProtKB · Q9Y624

Junctional adhesion molecule A

JAM1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9Y624
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F11R
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
JAM-A
EC number
—
Processing
Precursor
Secondary accessions
B7Z941
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

Cell junctionCell membraneMembraneTight junction

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

Host cell receptor for virus entryReceptor

Biological process

Host-virus interaction

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

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