ELISA Kit for Mesothelin (MSLN)

CAT: 0338-TRV-049514-01Size: 24 TestsDry Ice: NoHazardous: No

1 / 1

CAT#:0338-TRV-049514-01Size:24 Tests

Selected

24/48H Stock Items & 2 to 6 Weeks non Stock Items.

Quick Request Actions

CAS Number

7732-18-5

Product Name Alternative

SMR; MPF; CAK1; Pre-pro-megakaryocyte-potentiating factor; Megakaryocyte-potentiating factor

UniProt

Q13421

Applications

Enzyme-linked immunosorbent assay for Antigen Detection.

Field of Research

Metabolic pathway

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mesothelin (MSLN) . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mesothelin (MSLN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mesothelin (MSLN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mesothelin (MSLN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Protocol

1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C;
8. Add 50µ L Stop Solution. Read at 450nm immediately.

Assay Performance Time

Sample Type

Serum, plasma, tissue homogenates and other biological fluids

Detection Range

0.156-10ng/mL

Precision

Intra-assay Precision (Precision within an assay) : 3 samples with low, middle and high level Mesothelin (MSLN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays) : 3 samples with low, middle and high level Mesothelin (MSLN) were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100 Intra-Assay: CV Inter-Assay: CV Inter-Assay: CV

Sensitivity

The minimum detectable dose of this kit is typically less than 0.056ng/mL

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Format

48T, 96T, 96Tx5, 96Tx10, 96Tx100

Specificity

This assay has high sensitivity and excellent specificity for detection of Mesothelin (MSLN) . No significant cross-reactivity or interference between Mesothelin (MSLN) and analogues was observed.

Product Name Synonym

Mesothelin

Method

Double-antibody Sandwich

Organism Species

Homo sapiens (Human)

Chemical Information

Water

Water is an oxygen hydride consisting of an oxygen atom that is covalently bonded to two hydrogen atoms. It has a role as an amphiprotic solvent, a mouse metabolite, a Saccharomyces cerevisiae metabolite, a member of greenhouse gas, an Escherichia coli metabolite and a human metabolite. It is an oxygen hydride, a mononuclear parent hydride and an inorganic hydroxy compound. It is a conjugate base of an oxonium. It is a conjugate acid of a hydroxide.

CAS Number	7732-18-5
PubChem CID	962
IUPAC Name	oxidane
Molecular Formula	H2O
Molecular Weight	18.015
XLogP	-0.5
Topological Polar Surface Area	1
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Heavy Atom Count	1
Formal Charge	0
Complexity	0

SMILES

InChI

InChI=1S/H2O/h1H2

InChIKey

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Chemical Structure

2D Structure

CAS: 7732-18-5

CID: 962

Formula: H2O

MW: 18.015

Interactive 3D Structure

Loading 3D structure...

View on PubChem Raw 3D data

Computed Properties

Property	Value
Molecular Weight	18.015
XLogP3	-0.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	0
Exact Mass	18.010564683
Monoisotopic Mass	18.010564683
Topological Polar Surface Area	1 Å²
Heavy Atom Count	1
Formal Charge	0
Complexity	0
Isotope Atom Count	0
Defined Atom Stereocenter Count	0
Undefined Atom Stereocenter Count	0
Defined Bond Stereocenter Count	0
Undefined Bond Stereocenter Count	0
Covalently-Bonded Unit Count	1
Compound Is Canonicalized	Yes

UniProtKB · Q13421

Mesothelin

MSLN_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q13421
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MSLN
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: D3DU65, Q14859, Q4VQD5, Q96GR6, Q96KJ5, Q9BR17, Q9BTR2, Q9UCB2, Q9UK57

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Cellular component

Cell membraneGolgi apparatusMembraneSecreted

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinGPI-anchorLipoproteinPhosphoprotein

Domain

Signal

Related Products

Catalog	Name
TRV-031176-01	Recombinant Mesothelin (MSLN)
TRV-030650-01	Recombinant Mesothelin (MSLN)
TRV-030146-01	Eukaryotic Mesothelin (MSLN)
TRV-043944-01	Eukaryotic Mesothelin (MSLN)
TRV-043940-01	Eukaryotic Mesothelin (MSLN)
TRV-040975-01	Recombinant Mesothelin (MSLN)
TRV-028333-01	Recombinant Mesothelin (MSLN)
TRV-025977-01	Active Mesothelin (MSLN)
TRV-063556	Multiplex Assay Kit for Mesothelin (MSLN)
TRV-047498-01	ELISA Kit for Anti-Mesothelin Antibody (Anti-MSLN)

Alternative Products

Catalog	Name
MBS702446-01	Human Mesothelin, MSLN ELISA Kit
MBS4501157-01	Mouse Mesothelin (MSLN) ELISA Kit
MBS4501156-01	Human Mesothelin (MSLN) ELISA Kit
EM23397	Mouse MSLN (Mesothelin) ELISA Kit
F1075	MSLN (Mesothelin) Porcine ELISA Kit
F1073	MSLN (Mesothelin) Human ELISA Kit
KLM5922	GENLISA™ Mouse Mesothelin (Msln) ELISA
MBS9373347	Plant Mesothelin (MSLN) ELISA Kit
RK14629	Rat Mesothelin (MSLN) ELISA Kit
EH0225	Human MSLN (Mesothelin) ELISA Kit