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Human DDX5 (DEAD Box Polypeptide 5) Microsample ELISA Kit

CAT: 0855-ELK6151MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6151MS-01Size:48 Tests
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Product Name Alternative
G17P1; HLR1; HELR; HUMP68; p68; Asp-Glu-Ala-Asp Box Polypeptide 5; Probable ATP-dependent RNA helicase DDX5
UniProt
P17844
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DDX5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DDX5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DDX5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DDX5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.063 ng/mL

UniProtKB · P17844

Probable ATP-dependent RNA helicase DDX5

DDX5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17844
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DDX5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.4.13
Processing
—
Secondary accessions
B4DLW8, B5BU21, D3DU32, E7ETL9, O75681, Q53Y61
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Biological process

Biological rhythmsmRNA processingmRNA splicingTranscriptionTranscription regulation

Cellular component

CytoplasmNucleusSpliceosome

Molecular function

HelicaseHydrolaseRNA-binding

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