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Human CGa (Chorionic Gonadotropin Alpha Polypeptide) Microsample ELISA Kit

CAT: 0855-ELK5432MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5432MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
FSHA; GPHA1; GPHa; HCG; LHA; TSHA; Thyroid Stimulating Hormone Alpha; Follicle-Stimulating Hormone Alpha Subunit; Luteinizing hormone alpha chain; Lutropin alpha chain
UniProt
P01215
Reactivity
Human
Field of Research
Tumor immunity; Endocrinology; Reproductive science; Genetic science; Hormone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CGa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CGa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CGa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CGa in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.056 ng/mL

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