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Human EFNA2 (Ephrin A2) Microsample ELISA Kit

CAT: 0855-ELK5128MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5128MS-01Size:48 Tests
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Product Name Alternative
ELF-1; EPLG6; HEK7-L; LERK6; EPH-related receptor tyrosine kinase ligand 6; HEK7 ligand
UniProt
O43921
Reactivity
Human
Field of Research
Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · O43921

Ephrin-A2

EFNA2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O43921
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EFNA2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O76020
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinGPI-anchorLipoprotein

Domain

Signal

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