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Human IREB2 (Iron Responsive Element Binding Protein 2) Microsample ELISA Kit

CAT: 0855-ELK4764MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4764MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IRP2; Iron regulatory protein 2
UniProt
P48200
Reactivity
Human
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IREB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IREB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IREB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IREB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.058 ng/mL

UniProtKB · P48200

Iron-responsive element-binding protein 2

IREB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P48200
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IREB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IRE-BP 2
EC number
—
Processing
—
Secondary accessions
A0A0A6YY96, A8KAC7, E1CJT9, H0YKU0, Q13095, Q1HE21, Q59FQ7, Q8WVK6, Q9UF17
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Ligand

4Fe-4SIronIron-sulfurMetal-binding

Coding sequence diversity

Alternative splicing

Cellular component

Cytoplasm

Disease

Disease variantNeurodegeneration

Molecular function

RNA-binding

PTM

Ubl conjugation

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