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Human CD27BP (CD27 Binding Protein) Microsample ELISA Kit

CAT: 0013-GTR18871596-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18871596-01Size:48 Tests
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Description
Human CD27BP (CD27 Binding Protein) Microsample ELISA Kit
Product Name Alternative
SIVA; SIVA1, Apoptosis-Inducing Factor; Apoptosis regulatory protein Siva
UniProt
O15304
Reactivity
Human
Field of Research
Immunology & Inflammation; Cell Biology
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.071 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CD27BP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD27BP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CD27BP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CD27BP in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

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