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Human MHCA (Major Histocompatibility Complex Class I A) Microsample ELISA Kit

CAT: 0855-ELK4527MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4527MS-01Size:48 Tests
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Product Name Alternative
HLA-A; HLAA; MHC-A; Leukocyte Antigen A; HLA class I histocompatibility antigen, A-31 alpha chain
UniProt
P04439
Reactivity
Human
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCA in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.119 ng/mL

UniProtKB · P04439

HLA class I histocompatibility antigen, A alpha chain

HLAA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04439
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HLA-A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B1PKZ3, O02939, O02954, O02955, O02963, O19509, O19546, O19598, O19605, O19606, O19619, O19647, O19673, O19687, O19695, O19756, O19794, O19795, O43906, O43907, O46874, O62921, O62924, O77937, O77938, O77964, O78073, O78171, O98009, O98010, O98011, O98137, P01891, P01892, P05534, P06338, P10313, P10314, P10315, P10316, P13746, P16188, P16189, P16190, P18462, P30443, P30444, P30445, P30446, P30447, P30448, P30449, P30450, P30451, P30452, P30453, P30454, P30455, P30456, P30457, P30458, P30459, P30512, P30514, P79505, P79562, P79563, Q09160, Q29680, Q29747, Q29835, Q29837, Q29838, Q29899, Q29908, Q29909, Q29910, Q30208, Q31623, Q5S3G1, Q65A82, Q8MHM1, Q8MHN9, Q95352, Q95355, Q95362, Q95377, Q95380, Q95IZ5, Q9BCN0, Q9BD15, Q9BD19, Q9GJE6, Q9GJE7, Q9GJE8, Q9MW42, Q9MY89, Q9MYA3, Q9MYA5, Q9MYC4, Q9MYE6, Q9MYE9, Q9MYG4, Q9MYG5, Q9MYI5, Q9TP25, Q9TPQ3, Q9TPR8, Q9TPX8, Q9TPX9, Q9TPY0, Q9TQ24, Q9TQE8, Q9TQE9, Q9TQF1, Q9TQF5, Q9TQF8, Q9TQF9, Q9TQG0, Q9TQG5, Q9TQG7, Q9TQH5, Q9TQI3, Q9TQK5, Q9TQM6, Q9TQN5, Q9TQP5, Q9TQP6, Q9TQP7, Q9UIN1, Q9UIN2, Q9UIP7, Q9UQU3, Q9UQU6, Q9UQU7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Adaptive immunityHost-virus interactionImmunityInnate immunity

Coding sequence diversity

Alternative splicing

Cellular component

Cell membraneEndoplasmic reticulumMembraneMHC I

PTM

Disulfide bondGlycoproteinPhosphoproteinSulfationUbl conjugation

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

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