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Human MHCE (Major Histocompatibility Complex Class I E) ELISA Kit

CAT: 0855-ELK10370-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10370-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCE in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E
UniProt
P13747
Reactivity
Human
Field of Research
Signal transduction; Tumor immunity; Infection immunity; Immune molecule; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MHCE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MHCE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MHCE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MHCE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.057 ng/mL

UniProtKB · P13747

HLA class I histocompatibility antigen, alpha chain E

HLAE_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P13747
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
HLA-E
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
E2G051, Q30169, Q6DU44, Q9BT83, Q9GIY7, Q9GIY8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Biological process

Adaptive immunityHost-virus interactionImmunityInnate immunity

Cellular component

Cell membraneGolgi apparatusMembraneMHC ISecreted

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

SignalTransmembraneTransmembrane helix

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