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Mouse MEPE (Matrix Extracellular Phosphoglycoprotein) Microsample ELISA Kit

CAT: 0855-ELK3641MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3641MS-01Size:48 Tests
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Product Name Alternative
OF45; Asarm Motif-Containing; Osteoblast/Osteocyte Factor 45
UniProt
Q8K4L6
Reactivity
Mouse
Field of Research
Tumor immunity; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MEPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MEPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MEPE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
30 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.47-30 ng/mL
Sensitivity
0.22 ng/mL

UniProtKB · Q8K4L6

Matrix extracellular phosphoglycoprotein

MEPE_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8K4L6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Mepe
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D6C6N6, Q3TYZ5, Q924I1
Protein keywords

Biological process

Biomineralization

Cellular component

Extracellular matrixSecreted

PTM

GlycoproteinPhosphoproteinProteoglycan

Technical term

Reference proteome

Domain

Signal

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