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Mouse LY75 (Lymphocyte Antigen 75) Microsample ELISA Kit

CAT: 0855-ELK3532MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3532MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CD205; LY75; CLEC13B; DEC-205; GP200-MR6; C-type lectin domain family 13 member B
UniProt
Q60767
Reactivity
Mouse
Field of Research
Signal transduction; CD & Adhesion molecule; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse LY75. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse LY75. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse LY75, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse LY75 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell Lysates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL

UniProtKB · Q60767

Lymphocyte antigen 75

LY75_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q60767
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ly75
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Ly-75
EC number
—
Processing
Precursor
Secondary accessions
B2RWW5, Q8C7T3, Q91XL8, Q9QUZ6
Protein keywords

Technical term

Direct protein sequencingReference proteome

PTM

Disulfide bondGlycoproteinPhosphoprotein

Biological process

Endocytosis

Ligand

Lectin

Cellular component

Membrane

Molecular function

Receptor

Domain

RepeatSignalTransmembraneTransmembrane helix

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