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Rat NSE (Enolase, Neuron Specific) Microsample ELISA Kit

CAT: 0855-ELK2589MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2589MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
UniProt
P07323
Reactivity
Rat
Field of Research
Enzyme & Kinase; Tumor immunity; Infection immunity; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NSE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.65 ng/mL

UniProtKB · P07323

Gamma-enolase

ENOG_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P07323
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eno2
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
—
Protein keywords

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

Cell membraneCytoplasmMembrane

Technical term

Direct protein sequencingReference proteome

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding

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