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Mouse NSE (Enolase, Neuron Specific) Microsample ELISA Kit

CAT: 0013-GTR18874212-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18874212-01Size:48 Tests
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Description
Mouse NSE (Enolase, Neuron Specific) Microsample ELISA Kit
Product Name Alternative
ENO2; Enolase 2; Gamma Enolase; 2-phospho-D-glycerate hydro-lyase; Neural enolase
UniProt
P17183
Reactivity
Mouse
Field of Research
Neuroscience; Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity
0.46 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NSE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NSE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NSE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NSE in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.78-50 ng/mL

UniProtKB · P17183

Gamma-enolase

ENOG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P17183
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Eno2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
4.2.1.11
Processing
—
Secondary accessions
—
Protein keywords

PTM

AcetylationHydroxylationIsopeptide bondPhosphoproteinUbl conjugation

Cellular component

Cell membraneCytoplasmMembrane

Technical term

Direct protein sequencingReference proteome

Biological process

Glycolysis

Molecular function

Lyase

Ligand

MagnesiumMetal-binding

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