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Human IL1a (Interleukin 1 Alpha) Microsample ELISA Kit

CAT: 0855-ELK2273MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2273MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL1 Alpha; IL1-A; IL-1α; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
UniProt
P01583
Reactivity
Human
Field of Research
Cytokine; Apoptosis
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.5 pg/mL

UniProtKB · P01583

Interleukin-1 alpha

IL1A_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P01583
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL1A
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-1 alpha
EC number
—
Processing
Precursor
Secondary accessions
Q53QF9, Q7RU02
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinLipoproteinMyristatePhosphoprotein

Molecular function

CytokineMitogenPyrogen

Cellular component

CytoplasmNucleusSecreted

Biological process

Inflammatory response

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