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Rat IL1a (Interleukin 1 Alpha) Microsample ELISA Kit

CAT: 0855-ELK1148MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1148MS-01Size:48 Tests
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Product Name Alternative
IL1 Alpha; IL1-A; IL-1α; IL-1a; IL1F1; Preinterleukin 1 Alpha; Hematopoietin-1; Pro-Interleukin-1-Alpha; Interleukin-1 Family Member 1
UniProt
P16598
Reactivity
Rat
Field of Research
Cytokine; Apoptosis
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL1a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL1a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6.1 pg/mL

UniProtKB · P16598

Interleukin-1 alpha

IL1A_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P16598
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il1a
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
IL-1 alpha
EC number
—
Processing
Precursor
Secondary accessions
Q546R9
Protein keywords

PTM

AcetylationGlycoproteinPhosphoprotein

Molecular function

CytokineMitogenPyrogen

Cellular component

CytoplasmNucleusSecreted

Biological process

Inflammatory response

Technical term

Reference proteome

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