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Mouse IL12A (Interleukin 12A) Microsample ELISA Kit

CAT: 0855-ELK2265MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2265MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IL-12A; p35; IL12-A; CLMF1; NFSK; NKSF1; Natural Killer Cell Stimulatory Factor 1; Cytotoxic Lymphocyte Maturation Factor 1; NF Cell Stimulatory Factor Chain 1
UniProt
P43431
Reactivity
Mouse
Field of Research
Cytokine; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL12A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL12A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL12A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL12A in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6 pg/mL

UniProtKB · P43431

Interleukin-12 subunit alpha

IL12A_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P43431
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Il12a
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IL-12A
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureReference proteome

Molecular function

CytokineGrowth factor

PTM

Disulfide bondGlycoprotein

Cellular component

Secreted

Domain

Signal

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