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Human IL11 (Interleukin 11) Microsample ELISA Kit

CAT: 0855-ELK2261MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2261MS-01Size:48 Tests
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Product Name Alternative
IL-11; AGIF; Adipogenesis Inhibitory Factor; Oprelvekin
UniProt
P20809
Reactivity
Human
Field of Research
Cytokine; Infection immunity; Rheumatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL11 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
6 pg/mL

UniProtKB · P20809

Interleukin-11

IL11_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P20809
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
IL11
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
IL-11
EC number
—
Processing
Precursor
Secondary accessions
B4DQV5, Q96EB4
Protein keywords

Technical term

3D-structurePharmaceuticalProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Molecular function

CytokineGrowth factor

Cellular component

Secreted

Domain

Signal

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