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Human ASM (Acid Sphingomyelinase) Microsample ELISA Kit

CAT: 0855-ELK1895MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1895MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
SMPD1; NPD; SMase; aSMase; Sphingomyelin Phosphodiesterase 1, Acid Lysosomal; Simply Sphingomyelinase
UniProt
P17405
Reactivity
Human
Field of Research
Enzyme & Kinase; Metabolic pathway; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ASM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ASM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ASM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ASM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P17405

Sphingomyelin phosphodiesterase

ASM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P17405
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMPD1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.4.12, 3.1.4.3
Processing
Precursor
Secondary accessions
A8K8M3, E9PKS3, P17406, Q13811, Q16837, Q16841
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Disease variantNeurodegenerationNiemann-Pick disease

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

GlycosidaseHydrolase

Biological process

Host-virus interactionLipid metabolism

Cellular component

Lipid dropletLysosomeSecreted

Ligand

Metal-bindingZinc

Domain

Signal

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