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Human NSMASE (Neutral Sphingomyelinase) Microsample ELISA Kit

CAT: 0013-GTR18872661-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872661-01Size:48 Tests
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Description
Human NSMASE (Neutral Sphingomyelinase) Microsample ELISA Kit
Product Name Alternative
SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2, Neutral Membrane; Lyso-platelet-activating factor-phospholipase C
UniProt
O60906
Reactivity
Human
Field of Research
Metabolism Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates and other biological fluids.
Sensitivity
0.065 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NSMASE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NSMASE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NSMASE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NSMASE in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · O60906

Sphingomyelin phosphodiesterase 2

NSMA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O60906
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SMPD2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.1.4.12
Processing
—
Secondary accessions
Q5TED1, Q9BWR3
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

Molecular function

Hydrolase

Biological process

Lipid metabolismSphingolipid metabolism

Ligand

MagnesiumMetal-binding

Domain

TransmembraneTransmembrane helix

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