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Rat CASP3 (Caspase 3) Microsample ELISA Kit

CAT: 0855-ELK1528MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1528MS-01Size:48 Tests
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Product Name Alternative
CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1
UniProt
P55213
Reactivity
Rat
Field of Research
Signal transduction; Enzyme & Kinase; Apoptosis; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CASP3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CASP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CASP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CASP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.126 ng/mL

UniProtKB · P55213

Caspase-3

CASP3_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P55213
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Casp3
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
CASP-3
EC number
3.4.22.56
Processing
Precursor
Secondary accessions
P70543, P97699, Q62993
Protein keywords

PTM

AcetylationPhosphoproteinS-nitrosylationUbl conjugationZymogen

Biological process

Apoptosis

Cellular component

Cytoplasm

Molecular function

HydrolaseProteaseThiol protease

Technical term

Reference proteome

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