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Rat IFNg (Interferon Gamma) Microsample ELISA Kit

CAT: 0855-ELK1133MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1133MS-01Size:48 Tests
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Product Name Alternative
IFN gamma; IFN-G; IFG; IFI; INFr; IFN; Immune Interferon
UniProt
P01581
Reactivity
Rat
Field of Research
Cytokine; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IFNg. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IFNg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IFNg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IFNg in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.63-1000 pg/mL
Sensitivity
5.7 pg/mL

UniProtKB · P01581

Interferon gamma

IFNG_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P01581
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ifng
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
IFN-gamma
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Antiviral defenseGrowth regulation

Molecular function

Cytokine

PTM

GlycoproteinPyrrolidone carboxylic acid

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal