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Mouse Interferon Gamma (IFN-γ) Microsample Fast ELISA Kit

CAT: 0013-GTR18853891-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18853891-01Size:48 Tests
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Description
Mouse Interferon Gamma (IFN-γ) Microsample Fast ELISA Kit
Product Name Alternative
IFNG, IFG, IFI, Type II Interferon, IFNγ
UniProt
P01580
Reactivity
Mouse
Target
IFN-γ
Field of Research
Infectious Disease & Virology; Immunology & Inflammation
Assay Type
Sandwich
Assay Performance Time
3H
Sample Type
Serum, Plasma and Tissue homogenate and other biological samples; Sample volume:25μL
Sensitivity
4.69 pg/mL
Notes
For research use only.
Applications Notes
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.
Dynamic Range
7.81-500pg/mL

UniProtKB · P01580

Interferon gamma

IFNG_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P01580
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ifng
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
IFN-gamma
EC number
—
Processing
Precursor
Secondary accessions
Q542B8
Protein keywords

Biological process

Antiviral defenseGrowth regulation

Molecular function

Cytokine

PTM

Glycoprotein

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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