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Mouse CD9 (CD9 antigen) ELISA Kit

CAT: 0855-ELK11315-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11315-01Size:48 Tests
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Product Name Alternative
CD9 antigen ; CD9
UniProt
P40240
Reactivity
Mouse
Field of Research
Signal transduction; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
300 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids
Detection Range
4.69-300 pg/mL
Sensitivity
1.34 pg/mL

UniProtKB · P40240

CD9 antigen

CD9_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P40240
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cd9
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q3U9W0
Protein keywords

Biological process

Cell adhesionFertilization

Cellular component

Cell membraneMembraneSecreted

PTM

Disulfide bondGlycoproteinLipoproteinPalmitate

Technical term

Reference proteome

Domain

TransmembraneTransmembrane helix

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