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Rat APOE (Apolipoprotein E) ELISA Kit

CAT: 0855-ELK11091-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11091-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOE in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Apo-E; AD2; Apoprotein; Alzheimer Disease 2 (E4-Associated, Late Onset
UniProt
P02650
Reactivity
Rat
Field of Research
Metabolic pathway; Cardiovascular biology; Hepatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat APOE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APOE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat APOE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APOE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
78.13-5000 ng/mL
Sensitivity
46.7 ng/mL

UniProtKB · P02650

Apolipoprotein E

APOE_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P02650
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoe
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
Apo-E
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

ChylomicronEndosomeExtracellular matrixHDLSecretedVLDL

Technical term

Direct protein sequencingReference proteome

PTM

GlycoproteinOxidationPhosphoprotein

Molecular function

Heparin-binding

Biological process

Lipid transportTransport

Ligand

Lipid-binding

Domain

RepeatSignal

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