Rat CFH (Complement Factor H) Microsample ELISA Kit

CAT: 0855-ELK10837MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10837MS-01Size:48 Tests
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Product Name Alternative
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
Reactivity
Rat
Field of Research
Metabolic pathway; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CFH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 μg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
7.82-500 μg/mL
Sensitivity
3.82 μg/mL

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