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Rat BDNF (Brain Derived Neurotrophic Factor) Microsample ELISA Kit

CAT: 0855-ELK10643MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10643MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Neurotrophin; Abrineurin
UniProt
P23363
Reactivity
Rat
Field of Research
Cytokine; Infection immunity; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat BDNF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.111 ng/mL

UniProtKB · P23363

Neurotrophic factor BDNF precursor form

BDNF_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P23363
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bdnf
Protein existence
2: Evidence at transcript level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
proBDNF
EC number
—
Processing
Precursor
Secondary accessions
Q53YL8
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

Growth factor

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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