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Mouse BDNF (Brain Derived Neurotrophic Factor) Microsample ELISA Kit

CAT: 0855-ELK10642MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10642MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Neurotrophin; Abrineurin
UniProt
P21237
Reactivity
Mouse
Field of Research
Cytokine; Infection immunity; Neuro science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BDNF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BDNF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BDNF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BDNF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.1 ng/mL

UniProtKB · P21237

Neurotrophic factor BDNF precursor form

BDNF_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P21237
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bdnf
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
proBDNF
EC number
—
Processing
Precursor
Secondary accessions
A2AII2, Q8CCH9
Protein keywords

Coding sequence diversity

Alternative splicing

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Technical term

Direct protein sequencingReference proteome

Molecular function

Growth factor

Cellular component

Secreted

Domain

Signal

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