Mouse Ab1-42 (Amyloid Beta Peptide 1-42) Microsample ELISA Kit

• Product Name Alternative:

  Amyloid beta 42 (ABeta 42) ; amyloid beta peptide 1-42; Aβ1-42, amyloid βpeptide 1-42

• Reactivity:

  Mouse

• Field of Research:

  Neuro science

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  1000 pg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  15.63 - 1000 pg/mL

• Sensitivity:

  4.34 pg/mL