Rat Ab1-42 (Amyloid Beta Peptide 1-42) ELISA Kit

CAT:

855-ELK4897-02

Size:

96 Tests

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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Ab1-42. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Ab1-42. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Ab1-42, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Ab1-42 in the samples is then determined by comparing the OD of the samples to the standard curve.
CAS Number: 9000-83-3
Gene Aliases: Amyloid beta 42(ABeta 42); amyloid beta peptide 1-42; Aβ1-42,amyloid βpeptide 1-42
Field of Research: Neuro science.
Assay Principle: Sandwich ELISA
Assay Performance Time: 3.5h
Standard: 1000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Detection Range: 15.63-1000 pg/mL
Sensitivity: 4.75 pg/mL
: true

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