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Rabbit PRL (Prolactin) ELISA Kit

CAT: 0855-ELK10614-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10614-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
LTH; Luteotropic Hormone
UniProt
Q28632
Reactivity
Rabbit
Field of Research
Endocrinology; Reproductive science; Genetic science; Hormone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.92 ng/mL

UniProtKB · Q28632

Prolactin

PRL_RABIT · Oryctolagus cuniculus

View on UniProt ↗
Primary accession
Q28632
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PRL
Protein existence
2: Evidence at transcript level
Organism
Oryctolagus cuniculus (Rabbit)
Taxonomy ID
9986
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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