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Mouse PRL (Prolactin) ELISA Kit

CAT: 0855-ELK10413-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10413-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
LTH; Luteotropic Hormone
UniProt
P06879
Reactivity
Mouse
Field of Research
Endocrinology; Reproductive science; Genetic science; Hormone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PRL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse PRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PRL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · P06879

Prolactin

PRL_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P06879
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Prl
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
PRL
EC number
—
Processing
Precursor
Secondary accessions
Q544X8
Protein keywords

PTM

Disulfide bondPhosphoprotein

Molecular function

Hormone

Biological process

Lactation

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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