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Human SIRT1 (Sirtuin 1) Microsample ELISA Kit

CAT: 0855-ELK10541MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10541MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
UniProt
Q96EB6
Reactivity
Human
Field of Research
Endocrinology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SIRT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SIRT1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.13 ng/mL

UniProtKB · Q96EB6

NAD-dependent protein deacetylase sirtuin-1

SIR1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96EB6
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
SIRT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
hSIRT1
EC number
2.3.1.286
Processing
—
Secondary accessions
Q2XNF6, Q5JVQ0, Q9GZR9, Q9Y6F0
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoproteinS-nitrosylationUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisBiological rhythmsDifferentiationHost-virus interactionMyogenesisTranscriptionTranscription regulation

Cellular component

CytoplasmMitochondrionNucleus

Molecular function

Developmental proteinTransferase

Ligand

Metal-bindingNADZinc

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