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Mouse GAL9 (Galectin 9) ELISA Kit

CAT: 0855-ELK10491-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10491-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
LGALS9; Ecalectin; Lectin, Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21
UniProt
O08573
Reactivity
Mouse
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GAL9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GAL9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GAL9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GAL9 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.051 ng/mL

UniProtKB · O08573

Galectin-9

LEG9_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O08573
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Lgals9
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Gal-9
EC number
—
Processing
—
Secondary accessions
O08572, Q3UKE5, Q99L83
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ChemotaxisImmunity

Cellular component

CytoplasmNucleusSecreted

Ligand

Lectin

Domain

Repeat

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