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Human PROC (Protein C) ELISA Kit

CAT: 0855-ELK10359-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10359-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROC in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
PRO-C; VKDPC; PC; Vitamin K Dependent Protein C; Inactivator Of Coagulation Factors Va And VIIIa; Autoprothrombin IIA; Blood coagulation factor XIV; Activation peptide
UniProt
P04070
Reactivity
Human
Field of Research
Enzyme & Kinase; Hematology; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PROC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PROC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PROC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PROC in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Plasma
Detection Range
1.57-100 ng/mL
Sensitivity
0.54 ng/mL

UniProtKB · P04070

Vitamin K-dependent protein C

PROC_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P04070
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PROC
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.69
Processing
Precursor
Secondary accessions
B4DPQ7, Q15189, Q15190, Q16001, Q53S74, Q9UC55
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Blood coagulationHemostasis

Ligand

Calcium

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidGlycoproteinHydroxylationPhosphoproteinZymogen

Disease

Disease variantThrombophilia

Domain

EGF-like domainRepeatSignal

Cellular component

Endoplasmic reticulumGolgi apparatusSecreted

Molecular function

HydrolaseProteaseSerine protease

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