Human eEF2 (Elongation Factor 2) ELISA Kit

CAT: 0855-ELK0230-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK0230-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human eEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human eEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human eEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human eEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
EF2; EF-2; EEF-2; SCA26
UniProt
P13639
Reactivity
Human
Field of Research
Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human eEF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human eEF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human eEF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human eEF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
19.3 pg/mL

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