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Mouse TFF2 (Trefoil Factor 2) ELISA Kit

CAT: 0855-ELK2774-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2774-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TFF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TFF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TFF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TFF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SML1; SP; Spasmolytic Protein 1; Spasmolysin; Spasmolytic polypeptide
UniProt
Q03404
Reactivity
Mouse
Field of Research
Tumor immunity; Gastroenterology; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TFF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TFF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TFF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TFF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.068 ng/mL

UniProtKB · Q03404

Trefoil factor 2

TFF2_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q03404
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Tff2
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
O08913
Protein keywords

PTM

Disulfide bond

Technical term

Reference proteome

Domain

RepeatSignal

Cellular component

Secreted

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