Recombinant Mouse IL-23

• Background:

  Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.

• Description:

  Recombinant Mouse Interleukin-23 is produced by our Mammalian expression system and the target gene encoding Val22-Ala196&Met23-Ser335 is expressed.

• Synonyms:

  SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

• UniProt:

  Q9EQ14&P43432

• Accession Number:

  Q9EQ14&P43432

• Endotoxin:

  Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells.The ED50 for this effect is 501.08 ng/ml.

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  19.7&35.8 KDa

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  18 & 40 - 55 KDa, reducing conditions

• Other Product Names:

  SGRF; IL-23p19; CLMF p40; IL-12 subunit p40; NKSF2

• CAS Number:

  9000-83-3