Recombinant Mouse IL-2

• Background:

  Interleukin 2 (IL 2), also termed T-cell growth factor, is a member of the cytokine family which includes IL-4, IL-7, IL-9, IL-15 and IL-21. Each member of this family has a four alpha helix bundle. IL-2 signals through the IL-2 receptor, a complex consisting of tree subunits, termed alpha, beta and gamma. The IL-2 R gamma is shared by cytokine receptors of all members of cytokine family. Mature mouse IL­2 shares 56% and 73% aa sequence identity with human and rat IL­2, respectively. IL-2 is produced by CD4+ T cell, CD8+ T cells, gamma δ T cells, B cells, dendritic cells and eosinophils, and plays a vital role in key function of the immune system, tolerance and immunity, primarily via its potent stimulatory activity for T cells.Thus, IL­2 may be a key cytokine in the natural suppression of autoimmunity.

• Description:

  Recombinant Mouse Interleukin-2 is produced by our E.coli expression system and the target gene encoding Ala21-Gln169 is expressed.

• Synonyms:

  aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF

• UniProt:

  P04351

• Accession Number:

  P04351

• Endotoxin:

  Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.

• Purity:

  Greater than 95% as determined by reducing SDS-PAGE.

• Bioactivity:

  Loaded Human IL-2RA-Fc (Cat#CJ78) on Protein A Biosensor, can bind Mouse IL-2 (Cat#CK24) with an affinity constant of 0.17 uM as determined in BLI assay.

• Reconstitution:

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Molecular Weight:

  17.4 KDa

• Shipping Conditions:

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Notes:

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight:

  17 KDa, reducing conditions

• Other Product Names:

  Aldesleukin; interleukin 2; interleukin-2; IL-2; IL2; T-cell growth facter; T cell growth factor; TCGF

• CAS Number:

  9000-83-3